Is it OK to freeze cells in the MR1+TCEP at -80 °C? I couldn't find freezing recommendations in the user manual for the NucleoMag RNA kit.
Customer can store samples in Lysis Buffer MR1 after disruption at -70 °C for up to one year (we recommend snap freezing). TCEP can also be added before freezing. Usually we recommend the following Handling, preparation, and storage of starting materials : • Use freshly harvested sample for immediate lysis and RNA purification. • Submerge and store samples in [...]
Is NucleoZOL compatible with NucleoMag kits (Pathogen)?
Yes. Customers can follow the recommendations for the TRIzol purified samples specified in the manual: https://www.mn-net.com/media/pdf/38/b1/4c/Instruction-NucleoMag-Pathogen.pdf From a technical point of view TRIzol and NucleoZOL are quite similar. Nucleic acid purification [...]
Customer’s stool samples were collected in ithe Genotek Omnigen collection tubes (OMR-200). What are the recommendations for Genotek Omnigen and NucleoSpin® DNA Stool kit?
We recommend removing the stabilization solution from the stool sample by centrifugation as this stabilization solution is none lysing. Then proceed with the stool sample 180 mg and mix it with lysis buffer ST1 as described in the standard protocol. It is possible that the stabilization solution contains salts which are not compatible with the kit itself. Nucleic acid [...]
What is the diameter of the NucleoMag Magnetic bead particles?
The size of the NucleoMag Magnetic bead particles ranges between 1 to 3 microns. Nucleic acid purification [...]
Are NucleoMag Beads Silica-based?
No. Macherey Nagel Magnetic beads are non-silica. The NucleoMag beads are supermagnetic carboxylated particles. https://www.takarabio.com/products/nucleic-acid-purification" target="_blank Nucleic acid purification [...]
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