When considering drop-out rates, does FACS lead to a significant percentage of empty wells in 384-well plates?

When looking at drop-out rates, there are a few things we need to take into consideration. First, is the potential that the sorter may not successfully place a single cell in each well. From testing at our flow cytometry core facility, we expect less than 10 empty wells in each 384-well plate. Another thing to consider is negative controls. In our 384-well plates, we include three negative [...]

If you are using a 384-well plate later, why are you pooling samples for ribosomal depletion into batches of 96 instead of 384?

We are currently limited by the number of unique indexes we can add to the plates for our pools. As soon as we have access to a larger number of indexes, we will work on creating larger pools of libraries and getting this in even faster throughput.SMART-Seq Stranded [...]

If you are using a 384-well plate later, why are you pooling samples for ribosomal depletion into batches of 96 instead of 384?

We are currently limited by the number of unique indexes we can add to the plates for our pools. As soon as we have access to a larger number of indexes, we will work on creating larger pools of libraries and getting this in even faster throughput.SMART-Seq Stranded [...]

How many samples will the Takara Bio SMART-Seq Stranded Kit process with these adaptations for a 384-well plate?

With the SPT Labtech mosquito HV, we have accomplished a six-fold reduction in the original volumes. Ideally, you should be able to do six-fold more reactions than the original kit.SMART-Seq Stranded [...]

How many samples will the Takara Bio SMART-Seq Stranded Kit process with these adaptations for a 384-well plate?

With the SPT Labtech mosquito HV, we have accomplished a six-fold reduction in the original volumes. Ideally, you should be able to do six-fold more reactions than the original kit.SMART-Seq Stranded [...]