Is it OK to freeze cells in the MR1+TCEP at -80 °C? I couldn't find freezing recommendations in the user manual for the NucleoMag RNA kit.
Is it OK to freeze cells in the MR1+TCEP at -80 °C? I couldn't find freezing recommendations in the user manual for the NucleoMag RNA kit.
| Customer can store samples in Lysis Buffer MR1 after disruption at -70 °C for up to one year (we recommend snap freezing). TCEP can also be added before freezing. Usually we recommend the following Handling, preparation, and storage of starting materials : • Use freshly harvested sample for immediate lysis and RNA purification. • Submerge and store samples in NucleoProtect® RNA or similar stabilization solutions. Allow a complete permeation of the sample with the stabilization solution before freezing it. Remove excess stabilization solution from the sample prior to RNA isolation according to the stabilization solution user manual. • Flash freeze sample in liquid N2 immediately upon harvest and store at -70 °C. Frozen samples are stable up to 6 months. Mortar and pestle can be used to pulverize the sample in a frozen state. Make sure that the sample does not thaw prior to contact with lysis buffer. • Store samples in Lysis Buffer MR1 after disruption at -70 °C for up to one year, at 4 °C for up to 24 hours or at room temperature for up to several hours. Samples frozen in Lysis Buffer MR1 should be thawed slowly before starting with the isolation of RNA. Nucleic acid purification products |