What is In-Fusion Cloning?

In‑Fusion Cloning is a highly efficient, ligation-independent cloning method, based on the annealing of complementary ends of a cloning insert and linearized cloning vector. This technology ensures easy, single-step directional cloning of any gene of interest into any vector at any locus. In‑Fusion constructs are seamless, enabling translational reading frame continuity without any [...]

How does reprogramming method affect the application of DEF-CS system?

Depending on different reprogramming methods used, cells need to be cultured in different media that are compatible with the reprogramming method and then after reprogramming, cells are transferred to DEF-CS Stem cell [...]

What are the candidates for substitute pluripotency tests?

Candidate alternatives to the teratoma assay comprise the directed differentiation of pluripotent stem cells into organotypic cells, differentiation of cells in embryoid bodies, the analysis of pluripotency-associated biomarkers with high correlation to the teratoma forming potential of stem cells, predictive epigenetic footprints, or a combination of these technologies. Stem cell [...]

What are the substitute convenient tests for pluripotency?

Invitrogen Taqman hPSC Scorecard. Stem cell [...]

What are the reliable tests for differentiation?

Proof of cell differentiation: In principle, all of the body’s cell types can be produced from iPS cells in the Petri dish. Here, too, it is essential that cells from the three germ layers be cultivated and their functionality proven. Proof of chimera formation: Scientists inject the iPS cells into mouse embryos and prove that they are contained in the growing organism. The iPS [...]