What are the reliable tests for differentiation?
What are the reliable tests for differentiation?
| Proof of cell differentiation: In principle, all of the body’s cell types can be produced from iPS cells in the Petri dish. Here, too, it is essential that cells from the three germ layers be cultivated and their functionality proven. Proof of chimera formation: Scientists inject the iPS cells into mouse embryos and prove that they are contained in the growing organism. The iPS cells are usually marked with a fluorescence gene that makes them visible under the microscope as luminous tissue. Demonstrating the presence of iPS cells that have matured into cells of the germline is viewed as particularly important in that it proves that the cells can convey their genetic information to the next generation. |