Which types of lentiviral vectors are compatible with Lenti-X Packaging Single Shots?

We have not tested all vectors, but in principle, any HIV-1-based vector should be efficiently packaged using this system, resulting in high-titer lentivirus. For example, a ZsGreen1 cassette in the pLenti6/V5 vector (Thermo Fisher Scientific)—a vector that lacks the WPRE and cPPT/CTS sequence elements and utilizes self-inactivating (SIN) LTRs—yielded titers of >3 x 107 IFU/ml, as determined [...]

How consistent are packaging results by Lenti-X packaging Single Shots?

Eliminating variability during reaction setup leads to consistent transfections that consistently yield high viral [...]

What type of cells should be used for packaging?

Lenti-X packaging systems are optimized for use with Lenti-X 293T Cells, a clone selected at Takara Bio for its ability to generate high-titer lentivirus. HEK 293 and 293FT cells can be used but will produce lower titers of packaged lentiviral [...]

What are Lenti-X Packaging Single Shots(VSV-G)?

Lenti-X Packaging Single Shots (VSV-G) provide an extremely simple and consistent one-step method for producing high-titer VSV-G pseudotyped lentivirus. The single-tube format consists of individual vials containing lyophilized Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this [...]

Is it possible to use the GoStix Plus app in languages other than English?

Yes. Depending on the language settings for your mobile device, the GoStix Plus app will display text in English, Japanese, Mandarin, or Korean. To override your device settings and have the app display text in English, navigate to the "About" page and select [Reset language settings]. Please note that it may be necessary to select UTF-8 encoding in Excel to properly view characters for languages [...]