How do I correlate GV to IFU/ml or ng p24?

Lenti-X GoStix Plus-based titers can be used in a similar fashion to ELISA-based titers. In the example of Lenti-X ZsGreen1 lentivirus, the virus stock was prepared using the Lenti-X Packaging Single Shots lentiviral packaging system. Percentages of cells transduced (% transduced) were determined on HT1080 cells via FACS analysis. In addition, levels of lentiviral p24 from this virus stock were [...]

Which devices are supported by the GoStix Plus app?

The app works on all Apple iPhones from 6 onwards using the iOS 9 operating system or newer, and on Apple iPod Touches from 6 onwards. Android smartphones running Android v6 and later are also supported. Please note: the GoStix Plus app has not been validated for use with [...]

Where do I download the GoStix app?

You can download the GoStix Plus app from the App Store or Google Play. Search for "GoStix [...]

How accurately do GV titers correlate to infectious units (IFU)?

Data demonstrate a strong correlation between GV and IFU across varying concentrations of sample and varying lentiviral packaging systems. Lenti‑X GoStix Plus-based titers correlate with transduction titers across varying concentrations. Lenti‑X ZsGreen1 lentivirus was prepared using the Lenti‑X Packaging Single Shots lentiviral packaging system. The sample preparation was serially diluted and [...]

What is a GoStix Value (GV) titer?

The GoStix Plus app scans the GoStix cassette for the test (T) and control (C) bands, measures the intensity of each band, and then performs a calculation that generates a metric which we refer to as the GoStix Value (GV; equivalent to ng/ml p24). The intensity of the test band on the cassette increases with the amount of p24 protein in your lentiviral supernatant, which in turn correlates with [...]