How accurately do GV titers correlate to infectious units (IFU)?
How accurately do GV titers correlate to infectious units (IFU)?
Data demonstrate a strong correlation between GV and IFU across varying concentrations of sample and varying lentiviral packaging systems.
Lenti‑X GoStix Plus-based titers correlate with transduction titers across varying concentrations. Lenti‑X ZsGreen1 lentivirus was prepared using the Lenti‑X Packaging Single Shots lentiviral packaging system. The sample preparation was serially diluted and added to Lenti‑X GoStix Plus cassettes and densitometric analysis was performed. In addition, each dilution was titrated on HT1080 cells to determine actual IFU/ml. The IFU/ml for the unknown samples was calculated using a GV to IFU/ml ratio determined from a reference virus produced using the same packaging system and the same harvest time as the unknown sample. The IFU/ml calculated from the Lenti‑X GoStix Plus was then plotted against the actual IFU/ml. The result was a strong positive correlation between GV and Actual IFU/ml.
Lenti‑X GoStix Plus-based titers correlate with transduction titers across varying packaging systems. ZsGreen1 lentivirus was prepared using three different commercially available lentiviral vector and packaging systems. Samples for all preparations were diluted and added to Lenti‑X GoStix Plus cassettes and analyzed. In addition, each dilution was titrated on HT1080 cells to determine actual IFU/ml. The IFU/ml for each unknown sample was calculated using a GV (ng/ml p24) to IFU/ml ratio determined from a reference virus produced using the same packaging system and the same harvest time as the unknown sample. Results from GV calculation correlates well with actual IFU/ml from various systems.