What is p24 in Lentiviral titration kits?

Recombinant lentiviruses generated for research use contain major structural proteins, including envelope proteins, matrix proteins, and virus core proteins. p24 is a structural protein that comprises a majority of the capsid (or shell) of lentiviral particles, and is encoded by the gag gene (along with the nucleocapsid proteins, p6 and p7, and the matrix protein, p17). Lenti‑X GoStix Plus and [...]

What are the benefits of using Lenti-X GoStix Plus to measure lentiviral titer?

Lenti‑X GoStix Plus cassettes provide a fast and simple method for obtaining an accurate viral titer. Traditional methods for measuring titer such as qPCR, ELISA, or functional assays can be fairly lengthy and cumbersome, and are therefore not ideal for monitoring your supernatant to identify an optimal harvest time, or for testing multiple samples. Lenti‑X GoStix Plus enable you to obtain a [...]

Can I use the Unique Dual index kit from cat No R400745 with SMART-seq mRNA LP kit?

No - R400745 includes 5uM UDIs (Catalog numbers 631752 - 634756) . The Unique Dual Index Kit used in the SMART-Seq mRNA LP (with UMIs) kit has 12.5 uM UDIs, and the Iibrary construction protocol in the SMART-Seq mRNA LP (with UMIs) User Manual is written for 12.5 uM UDIs (Catalog numbers 634752 - 634756) Unique Dual Index Kit NGS-RNA [...]

Comparison between Takara RT-qPCR kits.

Comparison between Takara RT-qPCR kits. One Step RT-qPCR kits for Probe-Based assays Premix RT Enzyme PCR Enzyme Probe Dye-based RT reaction Volume Template XU0002 5X One Step PrimeScript™ III RT-qPCR Kit, no ROX, GPR 200 Rxns 5X PrimeScript III Takara [...]

What are the main features of adeno-associated viruses?

Adeno-associated virus (AAV) is a nonenveloped virus that belongs to the Parvovirus family of the Dependovirus genus. AAV is not thought to be pathogenic to humans and only replicates in the presence of a helper virus, such as adenovirus or herpesvirus. The capsid structural proteins determine the ability of the virus to infect tissues/cells; tissue-specificity is known to differ for various [...]