Can I purchase RCU buffer from 740910 NucleoSpin RNA Clean-up Maxi?

No. However, customer can buy buffer "Lysis Buffer RA1" which is comparable to buffer RCU. Also it is same volume as in the NucleoSpin RNA Clean up Maxi kit . Customer can proceed with buffer RA1 just like with the RCU concentrate and dilute it with 180 mL ethanol. I would not recommend to use more ethanol to dilute the buffer. The kit is tested and optimized to [...]

What are the dimensions of the NucleoBond® Xtra 96 Plates EF?

• The 96-well filter, binding or finalizer plate: The top skirt follow more or less the SBS-format length - 126 mm x width 84.1 mm. The height of the skirt is approx. 15.20 mm. The height of the complete plate including nozzles is approx. 38 mm (37.75 mm). The plates can be stacked with the nozzles into each other. The total height without [...]

Do you have any special recommendations for isolating viral RNA from breast milk?

Both milk and plasma are protein-rich fluids and can be treated as similar/ equivalent biological fluids with regard to their biochemical properties. Therefore we recommend the NucleoSpin RNA Virus Kit (REF 740956/.50/.250) for this application. The NucleoSpin RNA Virus kit is designed for highly pure viral nucleic acids from fluid biological samples, for example plasma or serum. [...]

Is the RLT buffer from Qiagen compatible with NucleoMag Virus? Customer is isolating Covid-19 virus from various samples stored in the RLT buffer.

Yes. The RLT can be used in combination with NucleoMag RNA: - Clear lysate: centrifuge at 5,600 x g for 5 min, transfer supernatant to a new tube. - Add 1 vol of 70% EtOH to supernatant, as per Qiagen protocol. - Add 28 µL B-Beads per 350 µl sample - Mix well (e.g shaking for 5 min) - Put on magnetic stand for 2 min. Remove supernatant - Dry beads for 5 min. [...]

Can I use the NucleoProtect reagent for collecting nasal swabs?

No. Most swabs retain too much of the RNA protect solution and this will interfere with RNA isolation. Therefore we don't recommend using the NucleoProtect reagent for swabs. Nucleic acid purification [...]