Is the RLT buffer from Qiagen compatible with NucleoMag Virus? Customer is isolating Covid-19 virus from various samples stored in the RLT buffer.
Is the RLT buffer from Qiagen compatible with NucleoMag Virus? Customer is isolating Covid-19 virus from various samples stored in the RLT buffer.
| Yes. The RLT can be used in combination with NucleoMag RNA: - Clear lysate: centrifuge at 5,600 x g for 5 min, transfer supernatant to a new tube. - Add 1 vol of 70% EtOH to supernatant, as per Qiagen protocol. - Add 28 µL B-Beads per 350 µl sample - Mix well (e.g shaking for 5 min) - Put on magnetic stand for 2 min. Remove supernatant - Dry beads for 5 min. - Proceed with step 4 of the NucleoMag RNA protocol (DNA digest) For NucleoMag Virus we would recommend the following: - Mix 200 µL sample in RLT buffer with 10 µl Proteinase K, 4 µl Carrier RNA and incubate for 10 min - Add 600 µl MV2 and follow the standard protocol for NM Virus. |