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  2. ProdApplication

ProdApplication

232 Articles
  • 362
  • 0

Can I use the kit if I have no idea what the substitution is?

  • 344
  • 0

Why should I buy the Guide-it CRISPR Genome-Wide sgRNA Library System?

  • 273
  • 0

What are some example applications of pooled whole-genome sgRNA library screens?

  • 314
  • 0

What should I do if purified protein fails to elute from TALON resin?

  • 367
  • 0

What is xTractor Buffer?

  • 247
  • 0

Can I elute my his-tagged protein from TALON resin with a histidine gradient?

  • 256
  • 0

Can nonphosphate-based buffer systems such as Tris or acetate buffers be used with TALON resin?

  • 244
  • 0

Is the performance of TALON resin affected by the temperature?

  • 284
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What should be done if the protein contains a tag in the middle of the sequence, i.e., not a terminal tag?

  • 254
  • 0

What should be done if a his-tagged protein is secreted from the cell into the cell culture medium?

  • 292
  • 0

How can the yield of purified HAT- or 6xHis-tagged fusion protein be increased?

  • 303
  • 0

How do the purification conditions used with TALON resin maximize biological activity more effectively than nickel-based resins?

  • 367
  • 0

How does TALON provide better purity than Ni-NTA resins?

  • 191
  • 0

What types of his-tagged proteins does TALON resin bind to?

  • 339
  • 0

For indexes in 96-well-plate format: can I generate just a few samples at a time?

  • 330
  • 0

Can libraries made with your DNA unique dual index kits be pooled with libraries made using TruSeq CD indexes?

  • 328
  • 0

Can I perform single-read runs and still obtain both index sequences?

  • 427
  • 0

Can I do paired-end sequencing with single-indexed libraries?

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