How does TALON provide better purity than Ni-NTA resins?
How does TALON provide better purity than Ni-NTA resins?
Each TALON reactive core is complexed with a cobalt ion that has a much higher selectivity for histidine tags than nickel ions. Only proteins containing adjacent histidines or specially positioned histidines are able to bind to TALON. In contrast, the spatial requirement for nickel-based resins is less strict, so using Ni-NTA resins often results in copurification of contaminants.