How much amplified DNA from the PicoPLEX Single Cell WGA Kit do I need for array and qPCR assays?
For microarrays, we recommend starting with the amount recommended in the instructions from the assay manufacturer and titrating if necessary to improve results even further. For PCR assays, we recommend using 5–200 ng of amplified PicoPLEX Single Cell WGA Kit library.DNA-seq [...]
How can I label PicoPLEX Single Cell WGA Kit products for microarrays?
Random-prime labeling and chemical labeling.DNA-seq [...]
Can the PicoPLEX Single Cell WGA Kit be used for probe-based qPCR assays and sequencing assays?
Yes, as long as the amplimers are less than 250 bp.DNA-seq [...]
Can the PicoPLEX Single Cell WGA Kit be used for gel-based STR genotyping?
The PicoPLEX Single Cell WGA Kit has not been optimized for gel-based STR genotyping.DNA-seq [...]
What makes the PicoPLEX Single Cell WGA Kit suited for SNP genotyping?
The PicoPLEX Single Cell WGA Kit is well suited for SNP genotyping because the same loci are reproducibly amplified between different cells. SNPs contained within well-represented regions can be accurately detected without the stochastic dropouts and noise associated with other WGA methods.DNA-seq [...]
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