What NGS application(s) can the PicoPLEX Single Cell WGA Kit be used for? Which applications are specifically recommended for this kit?

The PicoPLEX Single Cell WGA Kit can be used for: *Copy number variation (CNV) analysis: superior for oligonucleotide and array CGH *SNP genotyping *Mutation detection by PCR The PicoPLEX Single Cell WGA Kit particularly excels at copy number variation analysis.DNA-seq [...]

Is the product of PicoPLEX Single Cell WGA Kit technology single- or double-stranded?

The end product of the PicoPLEX Single Cell WGA Kit is a mixture of single- and double-stranded DNA. DNA-seq [...]

Can buffer control reactions be distinguished from single-cell reactions using PicoPLEX Single Cell WGA Kit?

Yes. The PicoPLEX Single Cell WGA Kit amplifies with single-copy sensitivity and high specificity.DNA-seq [...]

Does the PicoPLEX Single Cell WGA Kit reproducibly amplify genomic loci?

Yes, with about a 90% correlation coefficient for qPCR Ct data from replicate single-cell reactions.DNA-seq [...]

How robust is the PicoPLEX Single Cell WGA Kit process?

The PicoPLEX Single Cell WGA Kit has about a 90% amplification success rate with flow-sorted tissue culture cells, limited by the uncertainties of sorting rather than by amplification. Single cells always give a high, reproducible yield of amplified genomic DNA. Buffer controls should always give virtually no background.DNA-seq [...]