Is the SMARTer® Human BCR IgG IgM H/K/L Profiling Kit compatible with whole transcriptome profiling?

No - SMARTer® Human BCR IgG IgM H/K/L Profiling Kit has been designed and validated for BCR-specific amplification only.Immune profiling [...]

Can I use whole cells as input for the SMARTer® Human BCR IgG IgM H/K/L Profiling Kit?

No, SMARTer® Human BCR IgG IgM H/K/L Profiling Kit has not been validated for library construction using as input whole cells.Immune profiling [...]

What is the recommended sequencing depth for BCR profiling

The recommended sequencing depth for BCR profiling depends on the input RNA input quantity defining the sample complexity and is provided in the SMARTer® Human BCR IgG IgM H/K/L Profiling Kit User Manual - Table 4. Recommended Sequencing Depth for BCR Libraries Prepared from Human PBMC RNA or B Cell RNA.Immune profiling [...]

Is the SMARTer SMARTer® Human BCR IgG IgM H/K/L Profiling Kit suitable for BCR profiling using as input bulk whole cells?

SMARTer SMARTer® Human BCR IgG IgM H/K/L Profiling Kit has not been validated and is not recommended for bulk whole cells as input..Immune profiling [...]

Is the SMARTer SMARTer® Human BCR IgG IgM H/K/L Profiling Kit suitable for BCR profiling of single cells?

No, SMARTer® Human BCR IgG IgM H/K/L Profiling Kit is not compatible with single BCR cells.Immune profiling [...]