What is the source of the Control Total RNA included in the SMARTer Human TCR a/b Profiling Kit v2?

Control Total RNA, included in the SMARTer Human TCR a/b Profiling Kit v2 is Huma Blood Leukocyte RNA.Immune profiling [...]

Can I amplify TCR alpha and beta chains separately?

TCR alpha and beta chains can be amplified separately – each in a separate PCR2 reaction as described in the SMARTer Human TCR a/b Profiling Kit v2 User Manual V. Protocols - B. Protocol: TCR a/b Amplification and Sequencing Library Generation by Semi-Nested PCR - 2. PCR 2.Immune profiling [...]

Can I amplify TCR alpha and beta chains in one reaction?

TCR alpha and beta chains can be amplified in the same PCR2 reaction mix as described in the SMARTer Human TCR a/b Profiling Kit v2 User Manual V. Protocols - B. Protocol: TCR a/b Amplification and Sequencing Library Generation by Semi-Nested PCR - 2. PCR 2. If achieving equal coverage for each subunit is critical to your experiments, we recommend performing the reactions in PCR2 [...]

What is the number of maximal theoretical UMI combinations in the TCR-v2 sequencing reads?

The number of maximal theoretical UMI combinations is 4^12=16,777,216.Immune profiling [...]

What is the nature of the UMIs ?

Incorporated UMIs represent a random 12 nt sequence: NNN,NNN,NNN,NNN, (N=G,T,A,C)Immune profiling [...]