Does SMARTer smRNA-Seq library incorporate UMIs
No, SMARTer smRNA-Seq library does not incorporate UMIssmRNA-Seq [...]
What are the advantages of the SMARTer smRNA-Seq Kit for Illumina?
SMARTerĀ® smRNA-Seq Kit for Illumina has the highest sensitivity on the market compatible with as little as 1ng total RNA input, single post-PCR purification, high reproducibility across various input quantities of total RNA, ensuring a more accurate miRNA representation compared to the adaptor ligation-based method.smRNA-Seq [...]
Can I use SMARTer smRNA-Seq Kit for Illumina for RIP/CLIP/Ribosome profiling?
RNA templates obtained from methods such as ribosome profiling or CLIP/ RIP may require dephosphorylation of the 3'-end prior to to enable the initial polyadenylation step of the kit workflow, requiring the presence of intact 3'-OH.smRNA-Seq [...]
Can I validate SMARTer smRNA-Seq libraries using Fragment Analyzer?
SMARTerĀ® smRNA-Seq libraries can be validated by Advanced Analytical Fragment Analyzer High Sensitivity Small Fragment Kit (Advanced Analytical, Cat. No. DNF-477). smRNA-Seq [...]
Is SMARTer smRNA-Seq Kit for Illumina compatible with glycogen-precipitated RNA?
Glycogen may interfere with cDNA synthesis and should be avoided during RNA purification.smRNA-Seq [...]
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