What is the expected yield of cDNA generated by theSMART-Seq® mRNAKit for Sequencing ?

Depending on the RNA/ cell source and integrity, the expected yield of cDNA generated by the SMART-Seq® mRNA is [...]

What is the expected size of cDNA generated by the SMART-Seq® mRNA Kit for Sequencing?

Successful SMART-Seq cDNA synthesis and amplification should produce a cDNA library spanning 400–9,000 bp. The main peak should occur at approximately 2-2,500 bp.Ultra-low input NGS-RNA [...]

Is SMART-Seq mRNA kit compatible with bacterial RNA?

SMART-Seq mRNA cDNA synthesis is utilizing oligo(dT) primer for the first-strand cDNA synthesis, requiring for annealing the presence of poly(A) tails of high integrity eukaryotic RNA. Based on the above SMART-Seq mRNA kit is not compatible with bacterial RNA.Ultra-low input NGS-RNA products Care guide Caution [body] This care sheet provides general information only for handling Carolina™ [...]

Can I use whole yeast cells as input for direct SMART-Seq mRNA cDNA synthesis?

We do not recommend using yeast cells for direct SMART-Seq mRNA cDNA synthesis, since the yeast cell membrane may not be efficiently lysed under current optimized conditions.Ultra-low input NGS-RNA [...]

Can I use whole plant cells as input for direct SMART-Seq mRNA cDNA synthesis?

We do not recommend using plant cells for direct SMART-Seq cDNA synthesis, since the plant cell membrane may not be efficiently lysed under current optimized conditions.Ultra-low input NGS-RNA [...]