Can I freeze collected cells prior to the SMART-Seq cDNA synthesis?

Cells collected in one of the cell collection buffers recommended in the SMART-Seq mRNA User Manual can be frozen and stored at –80°C for up to two weeks.Ultra-low input NGS-RNA [...]

Can I use more than 1,000 cells as input for direct cDNA synthesis using SMART-Seq® mRNA kits?

Using more than 1,000 cells for direct cDNA synthesis with SMART-Seq® mRNA kits is not recommended, since the excess of cellular mass may inhibit the cDNA synthesisUltra-low input NGS-RNA [...]

What cell collection bufer is compatible with SMART-Seq mRNA cDNA synthesis directly from cells?

For cell collection buffer compatibility please, follow the recommendations of the SMART-Seq® mRNA Kit User Manual.Ultra-low input NGS-RNA [...]

What are the input requirements for the SMART-Seq® mRNA kit?

SMART-Seq mRNA kit utilizing oligo(dT) priming for the first-strand cDNA synthesis is compatible with 10pg-10ng high integrity total RNA (RIN?8) or 1-1,000 whole intact cells.Ultra-low input NGS-RNA [...]

What is the mechsmism of the SMART-Seq mRNA cDNA synthesis?

SMART-Seq mRNA kit utilizing oligo(dT) priming for the first-strand cDNA synthesis generates full-length double-stranded cDNA originated from full-length polyA RNAs. SMART Template Switching Oligo ensures an efficient cap-independent incoporporation of the 5’-end of mRNA contributing to uniform gene body coverage.Ultra-low input NGS-RNA [...]