Is there a nonstick wash buffer for washing single cells prior to using PicoPLEX NGS kits?

While this has not been validated at Takara Bio, some customers have reported successfully using polyvinyl alcohol (0.1% PVA, for example) in PBS to wash/collect their cells. Additionally, your washing/collecting buffer (PBS) must be Ca2+- and Mg2+-free.DNA-seq [...]

Should cells be washed before collection?

Yes. Cells should be washed to minimize extracellular DNA or growth media contaminants. We recommend washing in PBS (Ca2+-free, Mg2+-free, and BSA-free) and limiting carryover of wash buffer to less than 2.5 ul.DNA-seq [...]

What cell types have been successfully amplified by the PicoPLEX Single Cell WGA Kit?

Single blastomeres, polar bodies, trophoblasts, amniocytes, and cultured cells have been amplified successfully.DNA-seq [...]

What are the recommended DNA inputs for PicoPLEX NGS kits?

*1–10 human cells (e.g., blastomeres, polar bodies, trophoblasts, amniocytes, CTCs, or cultured cells) *1,000–10,000 bacterial cells *Intact or fragmented, single- or double-stranded DNA *Isolated DNA (15–60 pg of human DNA) NOTE: Inputs higher than 60 pg are possible, but require cycle optimization at certain steps *Input size over 500 bases *Sorted chromosomesDNA-seq products Care guide [...]

What are the strengths of PicoPLEX versus other WGA technologies?

The greatest advantage of PicoPLEX over other WGA technologies is the reproducibility obtained from cell to cell while using single cells. This technology also yields a very low background.DNA-seq [...]