Can I use scR-Probes, scZapR for the removal of rRNA sequences in other cDNA synthesis workflows or with pre-synthesized double-stranded cDNA?

No, scZapR -mediated removal of rRNA sequences has been designed to work exclusively as an integral part of the workflows of the SMART-Seq® Stranded [...]

What rRNA species are not removed by the ZapR

scR-Probes/ scZapR do not remove 5S, 5.8S rRNAs, rRNA precursors, mt rRNAs from mammalian species other than humans.SMART-Seq Stranded [...]

What is the mechanism of the removal of rRNA sequences within the workflow of the SMART-Seq® Stranded Kit?

A proprietary ZapR technology in conjunction with R-Probes, selectively removes mammalian rRNA (18S and 28S) and human mitochondrial rRNA (m12S and m16S) sequences from pre-amplified double-stranded cDNA.SMART-Seq Stranded [...]

Do I have to remove rRNA prior to the SMART-Seq® Stranded cDNA synthesis?

No, the removal of rRNA sequences from pre-amplified double-stranded cDNA is incorporated into the workflow of the SMART-Seq® Stranded Kit.SMART-Seq Stranded [...]

What are the input requirements for the SMART-Seq® Stranded Kit?

SMART-Seq® Stranded Kit is compatible with 10pg-10ng total mammalian RNA or 1-1,000 whole intact cells, utilizing a random priming for the first-strand cDNA synthesis and generating Illumina-ready library incorporating cDNA representing coding and non-coding RNAs with strand information.SMART-Seq Stranded [...]