History of Laminin

1. Laminin are membrane bound and previous purification methods involve harsh conditions. 2. Recombinant laminins can be expressed in mammalian cells and they retain the biological function of natural laminin. During expression, the membrane domains are truncated to ensure soluble expression. The alpha, beta and gamma subunits are cloned separately and co-trransfected into host cell. 3. [...]

What's the difference between Laminin-511 and Laminin-521

Laminin-511 is especially recommended for cell culture of murine embryonic stem cells or induced pluripotent stem cells while Laminin-521 is recommended for human pluripotent stem [...]

What is LIF?

LIF stands for Leukemia inhibitory factor, also known as differentiation inhibiting activity (DIA). It is an IL6 class cytokine that affects cell growth by inhibiting [...]

How to transfer iPS cells to DEF-CS

Cells grown in other culture systems: Undifferentiated human iPS cells maintained in other culture systems can be readily transferred to the DEF-CS system. It takes between 2 and 5 passages to adapt a cell line to the DEF-CS system. The standard DEF-CS culture protocol should be followed, although some modifications may increase the success of transfer: The cells may benefit from a higher [...]

Can cells grown in DEF-CS be differentiated into neurons?

Yes, it can be done. Stem Cell [...]