Can the DE cells be dissociated and frozen?

Yes, we provide a protocol for cryopreservation of the DE cells. Stem Cell [...]

Can the hiPS cell-derived hepatocytes be dissociated and reseeded?

Yes, we have developed a dissociation and reseeding protocol which can be made available as a customized solution. Please contact technical support. Stem Cell [...]

Can the DE cells be dissociated and reseeded?

Yes, the DE cells are dissociated and reseeded before the start of hepatocyte differentiation. Stem Cell [...]

Can I perform the hiPS cell hepatocyte differentiation in 3D?

No, the protocol and media only work for differentiation in 2D. Stem Cell [...]

Can I count the cells during hiPS cell-derived hepatocyte differentiation using an automated cell counter?

Starting with a homogenous culture and using the correct seeding density is key to success. Accurate cell counting is important during adaption of the hiPS cells to DEF-CS and for the differentiation. (View our Tips & Tricks page to learn why.) To start, we recommend counting in a hemocytometer/Bürker chamber since automated cell counters can give inaccurate numbers depending on the [...]