Can CYP enzyme activities be induced in hepatocytes derived with the Cellartis iPS Cell to Hepatocyte Differentiation System?

The hepatocytes display basal levels of activity of several important CYP enzymes. However, only a low level of CYP induction is observed. Stem Cell [...]

Do hiPS cell-derived hepatocytes that are generated with this system show formation of bile canaliculi?

We have not looked at formation of bile canaliculi in-house, but it has been observed by customers. Stem Cell [...]

Do hiPS cell-derived hepatocytes exhibit drug metabolism activity?

Yes, the hepatocytes have basal CYP enzyme activities, shown for CYP1A, CYP3A, CYP2C09, and CYP2C19 using LC/MS. Stem Cell [...]

How were CYP enzyme activities measured?

Liquid chromatography/mass spectrometry (LC/MS) was used to measure the formation of these specific metabolites: acetaminophen (CYP1A), 4-OH-Diclofenac (CYP2C9), OH-Bufuralol (CYP2D6), and 1-OH-Midazolam (CYP3A). To view the protocol, please see our tech note. Stem Cell [...]

Do hiPS cell-derived hepatocytes that are generated with this system express adult hepatocyte markers?

Yes. hiPS cell-derived hepatocytes generated from our system show expression of adult hepatocyte markers such as albumin, PXR, CYP1A3, CYP2C9, and CYP3A4. Stem Cell [...]