How often should I change the medium for cells grown in DEF-CS?

We recommend changing the medium every day; however, when the cell density is sparse, you can change the medium every other day. It is important to change the medium the day before and the day after passaging. Stem Cell [...]

What is the expected growth rate of the cells cultured in DEF-CS?

The typical generation time-the time needed for the cell population to double-of human iPS cells cultured with the DEF-CS system is 36 ± 5 hours. Stem Cell [...]

How many cells can be grown with the DEF-CS system?

One DEF-CS 500 kit (containing 500 ml basal medium) is sufficient to culture cells on approximately 600 cm2 at a harvesting density of 1.5-3.0 x 105 cells/cm2. This corresponds to 24 T25 culture flasks, eight T75 culture flasks, or four T150 flasks. Stem Cell [...]

Why doesn't the provided volume of DEF-CS GF-3 correspond to the provided volume of DEF-CS Basal Medium?

DEF-CS GF-1 and GF-2 are added to the basal medium when changing the media and during cell passaging. DEF-CS GF-3 is added to the basal medium only when passaging cells, not during each media change, therefore less volume is needed. Stem Cell [...]

Does the DEF-CS basal medium contain antibiotics?

Yes, it contains penicillin and streptomycin. Stem Cell [...]