What enzyme is used to passage cells cultured in DEF-CS?

The DEF-CS system employs enzymatic single-cell passaging and seeding. The system is optimized for use with TrypLE Select Enzyme (Cat. No. 12563029, Life Technologies). Other digestive enzymes may be used with optimization. Stem Cell [...]

What is the composition of the DEF-CS medium?

DEF-CS Basal Medium is serum free and is not feeder conditioned, however its composition is proprietary. The kit also includes three media additives and a matrix solution; the compositions of these are also proprietary. Stem Cell [...]

What is included in the DEF-CS system?

All reagents for human iPS cell culture are supplied, including three media additives and a matrix solution used to coat culture vessels for cell attachment. Enzymes and buffers for passaging are not included, but we recommend using TrypLE Select Enzymes (Cat. No. 12563029, Life Technologies). (Other enzymes can be used but require optimization.) Stem Cell [...]

What are the characteristics of human iPS cells cultured with the DEF-CS system?

Human iPS cells cultured with the DEF-CS system are typically 98% Oct-4 positive, as determined by flow cytometry. They also express common pluripotency markers, including the transcription factors Oct-4 and NANOG and the surface markers SSEA-4, TRA-1-60, and TRA-1-81. The cells do not express the differentiation marker SSEA-1. Cells grown in the DEF-CS system have been shown to differentiate in [...]

Can you culture Cellartis enhanced hiPS-HEP cells as 3D spheroids?

Yes, we have successfully generated 3D spheroids using ultra-low attachment plates. Spheroids can be maintained for 3–4 weeks in culture. Stem Cell [...]