Can CYP enzyme activities be induced in Cellartis enhanced hiPS-HEP cells?

The cells have basal CYP enzyme activities that are measured during quality control and presented in the Certificate of Analysis. Minimal CYP induction is observed in Cellartis enhanced hiPS-HEP cells in 2D culture. Stem Cell [...]

Do Cellartis enhanced hiPS-HEP cells express phase II enzymes?

Yes. In our tests, Cellartis enhanced hiPS-HEP cells showed mRNA expression of UGT1A1 and UGT2B7 at similar or higher levels than cryopreserved human primary hepatocytes. Enhanced hiPS-HEP cells displayed phase II enzyme activities, e.g., UGT and sulfotransferase activities, at similar or higher levels as in cryopreserved human primary hepatocytes cultured for 24 hours, as measured by LC/MS.Stem [...]

Do Cellartis enhanced hiPS-HEP cells express transporter proteins?

Yes. In our tests, Cellartis enhanced hiPS-HEP cells derived from ChiPSC12, ChiPSC18, and ChiPSC22 expressed several transporters: NTCP, OCT1, OATP1B1, and OATP1B3 (uptake, Panel A); plus MRP2, MDR1, and BSEP (efflux, Panel B). Some transporters were expressed at similar levels as cryopreserved human primary hepatocytes (hphep); other transporters were expressed at lower levels than hphep. mRNA [...]

What is the difference between a Cellartis enhanced hiPS-HEP v2 kit and the older version?

With Cellartis enhanced hiPS-HEP v2 kits, we are fulfilling a need for longer-lasting, functional, and mature hiPS cell-derived hepatocytes as a better alternative to primary and tumor-based cell models. Cellartis enhanced hiPS-HEP v2 kits contain the same high-performance Cellartis enhanced hiPS-HEP cells as the original kits. Both versions also contain hepatocyte thawing medium, coating, [...]

How soon after thawing Cellartis enhanced hiPS-HEP cells can I begin testing?

In our tests, high and constant CYP enzyme activities, plus albumin and urea secretion, were detected at Days 4–19 post-thawing. CYP activity (top panel) was measured by LC/MS at 4, 12, and 19 days post-thawing. Albumin and urea secretion (bottom panel) was measured by ELISA at 2, 4, 12, and 20 days post-thawing. Both were compared to human primary hepatocytes (hphep) after 20 (top) or 24 [...]