Why is Tet system approved fetal bovine serum necessary?

With the greatly increased sensitivity of the Tet-On 3G systems, it is more important than ever that the serum you use for your studies is tetracycline-free. Only Takara Bio performs actual inducibility tests on a sensitive Tet-inducible cell line in order to provide an absolute guarantee that your serum is tetracycline-free. Why is this necessary? Cattle are routinely fed tetracyclines to guard [...]

What are the viral delivery systems of Takara Tet system technology

We offer three types of viral delivery for Tet-inducible gene expression: 1. Lenti-X Tet-On 3G systems -Highest performing inducible lentivirus systems -Tightest control of gene expression -Each system includes an optimized, fourth-generation packaging mix designed to produce high-titer lentivirus 2. Retro-X Tet-On 3G system -Low background, high induction levels -Includes the GP2-293 [...]

Using the Tet system in transgenic mice

Conditional mouse models are made by crossing two transgenic mouse lines: one expressing the Tet-On transactivator under the control of a tissue-specific promoter, and the other containing the gene of interest cloned behind a TRE promoter. Expression of the gene under study in the double-transgenic progeny is controlled by the presence or absence of Dox in the drinking water. Hundreds of [...]

Tet-One or Tet-On 3G: which system should I choose?

In terms of technology, the Tet-One and Tet-On 3G systems are essentially identical. Both activate expression in the presence of doxycycline, using the Tet-On 3G transactivator to activate expression from a TRE3G promoter. For most applications, the Tet-One system is the best choice because it provides all components on a single vector, making it far easier to set up tightly controlled [...]

What's inducible coexpression and what are the advantages?

We offer two types of Tet-inducible gene coexpression. Both types give you a choice of expressing a target gene and a reporter gene, or two target genes. Bidirectional Tet-On 3G systems -Bidirectional vectors contain a specialized TRE3G promoter (PTRE3G-BI) that allows for inducible, simultaneous, and equivalent expression of two transgenes. Bidirectional vectors allow you to monitor expression [...]