What's the mechanism of Tet-inducible expression?

Each Tet-On 3G inducible gene expression system includes two core elements: 1. The inducible PTRE3G promoter, which provides the lowest basal expression of any tetracycline-inducible promoter in the absence of bound transactivator. PTRE3G retains very strong induced expression when activated by transactivator binding. 2. The Tet-On 3G transactivator, which binds to and activates expression from [...]

Does Xfect Protein Transfection Reagent affect cell viability?

No, as demonstrated when we performed a WST-1 assay to compare the effects on HeLa cell proliferation of Xfect Protein Transfection Reagent and Product P (a popular lipid-based protein transfection technology). The Xfect reagent did not affect cell proliferation compared to untreated cells, while Product P decreased cell proliferation by more than 30%. When we used Xfect Protein Transfection [...]

Does Xfect Protein Transfection Reagent deliver a high amount of protein?

Yes, Xfect Protein Transfection Reagent delivers far more protein per cell than the leading protein transfection reagent (Product C), a peptide-based reagent, in a broad range of cell types. This was demonstrated when we compared the amounts of ?-galactosidase and fluorescent proteins delivered by each reagent. When we compared six different cell types, Xfect protein delivered 3- to 13-fold more [...]

Is the transfected protein active with the Xfect Protein Transfection Reagent?

Yes, the protein is active. Assays that measure enzyme activity (such as caspase-3 or ß-galactosidase) show that these proteins retain their activity after transfection with Xfect Protein Transfection Reagent, while fluorescent proteins retain their ability to fluoresce. Xfect Protein Transfection Reagent delivers up to 8X more active ß-galactosidase into HeLa cells than the leading competitor, [...]

What are the advantages of Xfect Transfection Reagent compared to other protein delivery technologies?

The two technologies most frequently used for protein delivery are based on lipids and cell-penetrating peptides. Transfection with lipid-based reagents tends to result in high cytotoxicity, while peptide-based reagents transfect with low efficiency. Xfect Protein Transfection Reagent offers the best advantages of both (i.e., it retains low cytotoxicity and delivers more protein to a higher [...]