What is the optimal cargo-to-reagent ratio and amount for transfection?

Using excess or insufficient amounts of cargo and reagent can have adverse effects on transfection efficiency. Refer to your product manual for the optimal cargo:reagent ratio and maintain the recommended ratio across any experimental scale and/or culture surface area.Transfection [...]

What are the optimal cell density and passage number for transfection?

The overall health of target cells at the time of transfection is a key factor for transfection efficiency. We recommend using a 60–75% confluent culture for plasmid transfection—this is when cells are optimally active, and the risk of reagent toxicity is low. Xfect products are highly efficient with little to no cytotoxicity, further increasing your chances of success.Cell culture quality [...]

Upon addition of AAV Extraction Solution B, the solution turned pink; is this a problem?

Depending on the sample, the solution may turn pink upon addition of AAV Extraction Solution B, but this color change does not affect extraction efficiency.AAV [...]

Is AAVpro Extraction Solution compatible with any AAV serotype?

It can be used regardless of the serotype. However, for some serotypes lower extraction efficiency is obtained as compared with traditional methods. For AAV5, we have found lower extraction efficiency with AAVpro Extraction Solution as compared with freeze-thaw methods, but the final viral solution had fewer contaminants when AAVpro Extraction Solution was used.AAV [...]

What are the advantages of the AAVpro kit in comparison to other conventional titration methods?

Conventionally, a region of the inserted sequence is used for quantification of AAV vector. With this method, it is necessary to design primers for each AAV vector. The AAVpro Titration Kit (for Real Time PCR) Ver.2 uses a common region of the AAV vector, the AAV2 inverted terminal repeat (ITR), as a target. If the ITR region of the vector is derived from AAV2 (as is the case for most commonly [...]