Which serotypes can be measured with the AAVpro Titration Kit (for Real Time PCR) Ver.2?

The primers in this kit are specific for the inverted terminal repeat (ITR) region of AAV2. Therefore, if the AAV vector being used includes the ITR region from AAV2, it is possible to measure titer regardless of the serotype.AAV [...]

What is standard yield of AAV particles using the AAVpro Purification Kit (All Serotypes)?

Standard yields using the AAVpro Purification Kit (All Serotypes): AAVpro Purification Kit Midi (All Serotypes), Cat# 6675, yields 4 x 1010 – 2 x 1012 vg/prep after extraction; and 1 x 1010 – 5 x 1011 vg/prep after purification AAVpro Purification Kit Maxi (All Serotypes), Cat# 6666, yields 2 x 1011 – 1 x 1013 vg/prep after extraction; and 5 x 1010 – 2.5 x 1012 vg/prep after purificationAAV [...]

For administration to animals, how should AAV particles be purified?

There are no definitive purity criteria for in vivo transduction. You can confirm AAV purity by SDS-PAGE analysis; there should only be three major bands (corresponding to AAV VP1, VP2, and VP3). AAV particles purified using the AAVpro Purification Kit (All Serotypes) have been used successfully for in vivo transduction in several animal species.AAV [...]

What number of cells can be used for AAV purification with the AAVpro Purification Kit (All Serotypes)?

The AAVpro Purification Kit (All Serotypes) enables purification of AAV particles from cells grown in up to five T225 cell culture flasks. AAVpro Purification Kit Midi (All Serotypes), Cat# 6675, works for All Serotypes, contains 4 purifications with each purfication applicable to either 1 T225 flask, 4 10-cm dish, or 1.5 15-cm dish. AAVpro Purification Kit Maxi (All Serotypes), Cat# 6666, [...]

What are the benefits of using the AAVpro Purification Kit (All Serotypes)?

Ultracentrifugation is commonly used for AAV purification. However, this method requires a high skill level and several days (2–3) to complete. The AAVpro Purification Kit (All Serotypes) provides a simple and fast (~4 hours) method for AAV particle purification of any serotype from virus-producing cells. Purified AAV can be used for in vivo studies.AAV [...]