How long, and at what temperature, should amplified PicoPLEX Gold Single Cell DNA-Seq libraries be stored at before additional amplification?

For best performance, unpurified, amplified libraries prepared using our DNA-seq kits should be kept at –20°C for no more than seven days before additional amplification and/or purification. Please refer to the user manual for your kit for additional instructions.DNA-seq [...]

Can a library be prepared without any PCR amplification?

PCR amplification is required for libraries prepared using our DNA-seq kits. Please refer to the user manual for your kit to obtain detailed information about library amplification step(s).DNA-seq [...]

What is the recommended fluorescent dye for real-time PCR monitoring?

EvaGreen fluorescent dye (EvaGreen Dye, 20X in water; Biotium Cat. No. 31000) is recommended because of its high sensitivity and low interference with the amplification chemistry of our DNA-seq kits.DNA-seq [...]

Can libraries created using your DNA-seq kits be used for microarray or PCR analyses?

Yes, the libraries created using our DNA-seq kits can be used for microarray and PCR analyses.DNA-seq [...]

Can your DNA-seq kits be used for methylation studies?

No, our DNA-seq kits have not been optimized for methylation analyses. Instead, we recommend our EpiXplore Meth-Seq DNA Enrichment Kit.DNA-seq [...]