Can all of the reagents in your DNA-seq kits be mixed by vortexing?

No, only buffers and nuclease-free water may be vortexed, and should then be spun down briefly prior to use. Enzymes should be mixed by gentle pipetting and then spun down briefly prior to use. Index Plates should be spun down briefly prior to use.DNA-seq [...]

What reagents not supplied are necessary to generate sequencing-ready libraries using your DNA-seq kits?

Following generation of libraries using our DNA-seq kits, the samples will need to be purified using Agencourt AMPure XP beads (Beckman Coulter, Cat. No. A63880). Consult your product’s user manual for a complete list of required materials.DNA-seq [...]

Can the DNA-seq kits be run on any thermal cycler?

Our DNA-seq kits require the use of a thermal cycler that can accommodate a 50-µl final reaction volume. Please consult the manual of your PCR instrument.DNA-seq [...]

Is a high-fidelity enzyme used in the amplification reaction of DNA-seq kits?

Yes, a high-fidelity, high-processivity, low-bias DNA polymerase is used in our DNA-seq kits.DNA-seq [...]

What is the shelf life of your DNA-seq kits?

In general, the shelf life of our DNA-seq kits is one year from date of receipt under proper storage conditions. Consult your product's Certificate of Analysis for specific shelf life information. DNA-seq [...]