Can the Guide-it SNP Screening assay be used to distinguish homozygotes from heterozygotes?

As our data shown, each assay performed with the kit provides a qualitative output rather than a quantitative measure of a substitution's frequency. Therefore, a single Guide-it SNP screening assay using the current single-color chemistry cannot be used to distinguish homozygous clones from heterozygous clones (see FAQ above, ""Could the Guide-it SNP Screening Kit be used for genotyping?"" for [...]

Can the protocol for the Guide-it SNP Screening Kit be adapted for use with 384-well plates?

We have not tested the kit protocol using 384-well plates; however, theorizing such an adaptation, the only modification we can foresee at this time is a reduction in reaction volumes. Please note that some troubleshooting may be required to ensure successful setup of the PCR reaction and enzymatic assay and to confirm that the resulting fluorescent signal is strong enough to be detected using [...]

What cell densities are suitable for analysis with the Guide-it SNP Screening Kit?

Clonal cell lines in a 96-well plate can have different growth rates, and therefore the protocol has been optimized for a wide range of cell densities (from 2 x 104 to 2 x 105 cells per well). However, if this assay is being performed for the detection of homologous recombination we recommend that the assay be performed with genomic DNA that has been purified using NucleoSpin Tissue Columns [...]

Which cell types have been analyzed using the Guide-it SNP Screening Kit? Can the kit be used for suspension cells?

The Guide-it SNP Screening Kit has been used successfully to analyze a variety of cell types, including cells grown in suspension (Jurkat cells) and adherent cells (fibroblasts, hiPSCs).Gene editing [...]

Do I need to use column-purified genomic DNA for the Guide-it SNP Screening Kit?

No. The cells are lysed with MightyPrep Reagent for DNA, and the supernatant from the lysate is used directly as template for the PCR to amplify the genomic target sequence without the need for extra purification steps.Gene editing [...]