What's the copy number of the pBE-S vector from B. Subtilis Secretory Protein Expression System?

*High (pMSE3: 200–250 copies) (Silbersack et al. 2006), *medium (pBE-S: 50 copies) (Leonhardt 1990), and *low (pHB201: 5 copies) (Bron et al. 1987, 1998) copy numbers.Protein purification [...]

What are the index adapter sequences after preparation using ThruPLEX DNA libraries?

Index 1 (i7) Read: 5'-CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGA*T*C*T-3' Index 2 (i5) Read: 5'-AATGATACGGCGACCACCGAGATCTACACNNNNNNNNACACTCTTTCCCTACACGACGCTCTTCCGA*T*C*T-3'Unique Dual Indexing [...]

For indexes in 96-well-plate format: can I generate just a few samples at a time?

As we recommend no more than 4 freeze/thaw cycles for indexes provided in a 96-well-plate format (DNA HT dual index kits, Cat. # R400660–R400663), we strongly recommend processing at least 24 samples at a time.Unique Dual Indexing [...]

Are the Takara Bio DNA unique dual indexes the same as the Illumina TruSeq UD or IDT for Illumina UD sequences?

Yes, our DNA unique dual indexes are the same as the Illumina TruSeq UD or IDT for Illumina UD sequences.Unique Dual Indexing [...]

Can libraries made with your DNA unique dual index kits be pooled with libraries made using TruSeq CD indexes?

We do not recommend mixing libraries made with different index kits. If you want to run different libraries made with different index kits, we recommend loading each library in a different lane, as running libraries made with different index kits in the same lane may compromise your results.Unique Dual Indexing [...]