Does LymphoONE medium contain any human-derived components?

Yes. LymphoONE medium contains highly purified pharmaceutical-grade human serum albumin.T-cell transduction and culture [...]

Does LymphoONE medium contain any animal-derived components?

No. LymphoONE medium is xeno-free, meaning that it does not include any animal-derived (nonhuman) components.T-cell transduction and culture [...]

Does LymphoONE medium contain antibiotics?

Yes, the version of LymphoONE medium that is currently available (Cat. #s WK552S & WK552) contains streptomycin. In the future, we will launch a GMP-grade version of the medium without antibiotics.T-cell transduction and culture [...]

How does LymphoONE medium perform with the addition of serum?

While LymphoONE medium was developed to enable efficient T-cell expansion in the absence of serum, researchers always have the option of supplementing the medium with serum or plasma in order to achieve greater proliferation. As demonstrated below by the data involving stimulation by IL-2, anti-CD3 mAb, and RetroNectin reagent, supplementation of LymphoONE medium with human serum results in [...]

Takara enzyme for multiplex PCR

We recommend TaKaRa Taq DNA Polymerase or Titanium Taq DNA Polymerase for multiplex PCR. View our tech note for more information about using Titanium Taq DNA Polymerase in multiplex PCR for high-throughput genotyping.PCR [...]