Takara Direct amplification from tissue

We recommend Terra PCR Direct Polymerase Mix for amplifying targets from crude extracts or directly from tissues. The recommended amounts of various tissues that should be used for direct PCR are listed below: -Treated blood: ?5 µl -Mouse tail: ?1 mm -Mouse ear: ?1.5 mm2 -Plant leaf: ?1.2 mm (diameter) -Paraffin-embedded tissue section: ?1–1.5 cm2PCR [...]

Takara enzyme for highest sensitivity

For the highest sensitivity, we recommend Titanium Taq DNA Polymerase or TaKaRa Ex Taq DNA Polymerase. Both polymerases have been used successfully for highly sensitive genotyping applications.PCR [...]

Takara enzyme for colony PCR

We recommend SapphireAmp Fast PCR Master Mix for colony PCR. This enzyme premix can tolerate a substantial presence of bacterial nucleic acids. Tracking dye and density reagent are included in the master mix, allowing the PCR reaction products to be loaded directly on an agarose gel for electrophoresis.PCR products Care guide Caution [body] This care sheet provides general information only for [...]

Takara solution for gel electrophoresis after PCR (e.g., genotyping screens)

We recommend EmeraldAmp GT PCR Master Mix. This PCR master mix contains a green gel loading dye and density reagent, making it easy to prepare PCR reaction mixtures that can be directly loaded on an agarose gel for electrophoresis after PCR. EmeraldAmp GT PCR Master Mix can amplify targets up to 10 kb in length, including targets that are GC- or AT-rich. Additionally, PCR products generated with [...]

Takara enzyme for Paraffin/FFPE sections

The Terra PCR Direct FFPE Kit can be used for crude DNA preparation and direct PCR amplification from paraffin-embedded tissue sections. If it is necessary to extract DNA from paraffin-embedded tissue first, TaKaRa DEXPAT Easy and TaKaRa DEXPAT Reagent enable quick, efficient DNA preparation, even from slides or samples that have been stored for years.PCR [...]