What is the storage temperature of TB Green® Premix Ex Taq™ (Tli RNase H Plus)?

TB Green Premix Ex Taq™ (Tli RNase H Plus) kits should be stored in the dark. The storage at -80°C is most recommended for long term storage. Once thawed, they should be stored at 4°C for up to 6 months.Real-time PCR [...]

What is the difference between RR390A (Premix Ex Taq™ (Probe qPCR)) and RR039A (Premix Ex Taq™ DNA Polymerase (Perfect Real Time) kits?

TaqMan assay only and contains Tli RNase H. The RR039A is optimized for both – TaqMan and TB Green assays and doesn’t contain Tli RNase H. The RR390A is perfect for cDNA templates after the RT reaction, while RR039A is recommended for both - genomic and cDNA templates.Real-time PCR [...]

What is the role of Tli RNase H in qPCR reaction?

Tli RNase is a thermostable RNase H enzyme that allows to completely remove RNA in RNA/DNA duplexes that form after the first-strand cDNA synthesis. This exposes primer binding sites, especially GC-rich regions of cDNA more efficiently to PCR primers. The Tli RNase reduces template complexity by RNA degradation and cuts extension time.Real-time PCR [...]

Which SYBR Premix should I choose – RR420 or RR820? What is the difference between these premixes?

RR420 is recommended for higher sensitivity, lower Cq values, higher yield and longer templates (< 570 bp). RR820 is provided with an optimized buffer for increased specificity when primer design is suboptimal; reduces formation of primer dimers and gives robust amplification of templates up to 73% of GC content.Real-time PCR [...]

Can you mix the ROX™ reference dyes and TB Green® Premix Ex Taq™ to help avoid pipetting errors?

The ROX™ Reference dye I can be premixed. Add 40 ul of ROX™ to 1 ml of the TB Green® Premix Ex Taq™ and store at -20°C (protect from light). Use this solution within one month for best performance. The ROX™ Reference dye II should not be premixed prior to reaction assembly.Real-time PCR [...]