What is the composition of TB Green® Premix Ex Taq™?

TB Green® Premix Ex Taq contains Ex Taq™ Hot Start DNA Polymerase, buffer, dNTP mix, Mg2+ and TB Green®. The Mg2+ and TB Green® Green I concentrations are proprietary.Real-time PCR [...]

Why is the amplicon length so limited in qPCR?

Shorter amplicons have higher amplification efficiency and more likely to be completely denatured during the denaturing step, making the annealing of primers and probes more efficient.Real-time PCR [...]

What PCR product size is optimal for real-time PCR?

A size range of 80-150 bp is generally recommended for qPCR amplification, although sizes up to 300 bp are possible.Real-time PCR [...]

How much ROX is needed for each instrument? Why are the ROX concentrations different?

The amount of the ROX passive reference dye needed can vary depending on the instrument optics. More ROX dye may be needed with real-time PCR instruments which use an argon laser as the light source or which have excitation filters not optimal for ROX dye.Real-time PCR [...]

What is ROX used for?

ROX is a passive reference dye that can be used for normalizing against non-PCR related fluorescence signal variation. ROX does not take part in the qPCR reaction and its fluorescence remains constant during the PCR reaction.Real-time PCR [...]