What is the number of maximal theoretical UMI combinations in the sequencing reads generated by the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

The number of maximal theoretical UMI combinations is 4^8=65,536.Stranded RNA-seq for mammalian [...]

What is the nature of Unique Molecular Identifiers (UMI) incoporated into the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico libraries?

Incorporated UMIs represent a random 8 nt sequence: NNN,NNN,NN (N=G,T,A,C)Stranded RNA-seq for mammalian [...]

Can I use a software other than Cogent NGS Analysis Pipeline for the analysis of the sequencing reads generated by the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

If not using Cogent NGS Analysis Pipeline, follow thenucleotide trimming recommendations of the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian User Manual - Appendix C: Sequencing Guidelines - A. Sequencing Analysis GuidelinesStranded RNA-seq for mammalian [...]

What software should I use for the analysis of the sequencing reads generated by the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

Takara Bio USA offers free-of-charge Cogent NGS Analysis Pipeline Software referenced in the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian User Manual and available oline for download.Stranded RNA-seq for mammalian [...]

What is the advantage of incoporation of Unique Molecular Identifiers (UMI)?

Iincorporated Unique Molecular Identifiers (UMI) eliminating PCR and sequencing errors, reduce the number of duplicates and ensure a greater confidence in transcript identification, allowing to determine rare transcripts and transcript isoformsStranded RNA-seq for mammalian [...]