What is the advantage of using Unique Dual Indexes ?

Unique Dual Indexes are preferred for Illumina sequencers with patterned flow cells to avoid a potential index hopping - an incorrect assignment of libraries to a different index.Stranded RNA-seq for mammalian [...]

Can I use indexing primers from vendors other than Takara Bio USA?

Indexing primers from vendors other than Takara Bio USA, are not compatible with the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian, since within the workflow of the SMARTer Stranded Total RNA-Seq Kit v3 - Pico , PCR incorporation of indexing primers requires proprietary overlaps with the first-strand cDNA template as shown in the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico [...]

Can I use indexing primers from vendors other than Takara Bio USA?

Indexing primers from vendors other than Takara Bio USA, are not compatible with the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian, since within the workflow of the SMARTer Stranded Total RNA-Seq Kit v3 - Pico , PCR incorporation of indexing primers requires proprietary overlaps with the first-strand cDNA template as shown in the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico [...]

Can I use indexing primers from vendors other than Takara Bio USA?

Indexing primers from vendors other than Takara Bio USA, are not compatible with the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian, since within the workflow of the SMARTer Stranded Total RNA-Seq Kit v3 - Pico , PCR incorporation of indexing primers requires proprietary overlaps with the first-strand cDNA template as shown in the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico [...]

If RNA fragmentation is not required, what is the purpose of heating RNA at 720C?

The 72°C incubation step is performed in the absence of Mg2+. This avoids fragmentation but permits the relaxation of RNA secondary structure and random primer annealing.Stranded RNA-seq for mammalian [...]