What method of RNA fragmentation is used in the SMARTer Stranded Total RNA-Seq Kit v3 - Pico workflow?

RNA is heat-fragmented in the presence of Mg2+ (included in the 5X First-Strand Buffer). Fragmentation is then stopped by placing the reaction mixture on ice for 2 minutes.Stranded RNA-seq for mammalian [...]

Do I need to use a PhiX DNA spike-in during the sequencing of the libraries, generated by the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

Illumina recommends the systematic inclusion of ~1% PhiX to help assess run performance and troubleshooting. For Illumina sequencers with patterned flow cells, include 10% PhiX spike-in. Follow Illumina guidelines on how to denature, dilute, and combine a PhiX control library with your own pool of libraries.Stranded RNA-seq for mammalian [...]

What is the source of the Control Total RNA included in the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

Control Total RNA, included in the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian, is Human Brain Total RNA.Stranded RNA-seq for mammalian [...]

What RNA purification kits are recommended for compatibility with the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

We recommend the NucleoSpin RNA XS and NucleoSpin totalRNA FFPE XS kits offering on-column DNAse I treatment as well as optional DNAse I treatment of RNA eluate, followed by column purification . To ensure a complete removal of DNA, essential for the highly sensitive SMARTer Stranded Total RNA-Seq Kit v3 - Pico cDNA synthesis, it is advisable (if feasible), to perform an additional DNAse I [...]

What buffer should I collect cells in for compatibility with direct SMARTer Stranded Total RNA-Seq Kit v3 Pico cDNA synthesis?

SMARTer Stranded Total RNA-Seq Kit v3 Pico cDNA synthesis has been validated with cell collected or resuspended in Ca/Mg-free PBS. The final volume of collected cells should not exceed the sample input volume recommended in the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian User Manual.Stranded RNA-seq for mammalian [...]