Can I perform additional amplification of libraries prepared with the ThruPLEX DNA-Seq Kit if the concentrations or yields of the libraries are insufficient?

Yes, ThruPLEX DNA-Seq libraries can be further amplified without adding extra reagents after storage (4°C for up to 6 hours, or –20°C for up to 7 days). Please refer to the ThruPLEX DNA-Seq Kit User Manual for the library processing workflow and instructions on performing additional amplification cycles.DNA-seq [...]

Can the ThruPLEX DNA-Seq Kit be used without a real-time PCR machine? If so, how do I know when to stop my run?

Yes, the ThruPLEX DNA-Seq Kit can be used even if a lab does not have access to a real-time PCR machine. Please refer to the ThruPLEX DNA-Seq Kit User Manual, which contains a table that provides a guide for selecting the number of PCR cycles based on the DNA input amount used.DNA-seq [...]

Is it necessary to use indexing reagents for ThruPLEX DNA-Seq Kit? If so, which indexes should be used together for multiplexing?

Yes, it necessary to use indexing reagents, which must be purchased separately. They are supplied in tubes or a microplate and consist of amplification primers containing Illumina-compatible indexes, which must be used in the ThruPLEX DNA-Seq Library Amplification Reaction.DNA-seq [...]

What is the recommended DNA input range for the ThruPLEX DNA-Seq Kit?

The ThruPLEX DNA-Seq Kit has been designed to amplify an input range of 50 pg to 50 ng. The input amount depends on the desired level of complexity needed and the application.DNA-seq [...]

Where can I find information about a kit for preparing randomly fragmented genomic DNA for use with the ThruPLEX DNA-Seq Kit?

Our DNA Fragmentation Kit can be used to prepare randomly fragmented genomic DNA for use with the ThruPLEX DNA-Seq Kit by following the instructions in the DNA Fragmentation Kit User Manual.DNA-seq [...]