Can I use as input less than 250pg total RNA?

Lower RNA input quantities (0.1ng) may result in a higher duplicate rate due to the low complexity of the input RNA sample.Stranded RNA-seq for mammalian [...]

Can I use as input larger than 10ng total RNA?

SMARTer Stranded Total RNA-Seq Kit v2 Pico input Mammalian has been validated with up to 50ng total RNA. Depending on RNA source, larger RNA input quantities (50ng) may contribute to a higher% of rRNA sequences in the final SMARTer Stranded Total RNA-Seq Kit v2 – Pico library.Stranded RNA-seq for mammalian [...]

How efficient is ZapR-mediated removal of rRNA sequences?

ZapR-mediated removal of rRNA sequences may vary depending on RNA source and integrity. Compromised RNA may retain a higher percent of rRNA sequences in the final SMARTer Stranded v2 Pico library.Stranded RNA-seq for mammalian [...]

What is the source of the Control Total RNA included in the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian?

Control Total RNA, included in the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, is Human Brain Total RNA.Stranded RNA-seq for mammalian [...]

Can I purchase separately R-Probes v2, ZapR v2, ZapR Buffer(10X)?

R-Probes v2, ZapR v2, ZapR Buffer(10X) are not available as separate items, but only as components of the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian.Stranded RNA-seq for mammalian [...]