What Covaris shearing specifications should be used to fragment the DNA prior to ThruPLEX DNA-Seq Kit usage?

Covaris-recommended settings should be used to obtain the desired template fragment size. Please refer to the Covaris website for additional guidance.DNA-seq [...]

What total amount of DNA is needed for Covaris shearing, and what type of buffer volumes are used during the shearing?

Successful shearing has been accomplished with 2–50 ng of DNA in 50–130 µl of TE or low-TE buffer. If you are working with smaller amounts of DNA, see the ""Low-volume DNA shearing for ThruPLEX library prep"" tech note.DNA-seq [...]

What are the options available for DNA fragmentation for ThruPLEX DNA-Seq Kit?

DNA fragmentation can be accomplished either mechanically (e.g., Covaris, Bioruptor) or enzymatically (e.g., our DNA Fragmentation Kit).DNA-seq [...]

Is DNA fragmentation necessary for ThruPLEX DNA-Seq Kit?

Yes. If the DNA is >1 kb in size, it must be fragmented prior to use with the ThruPLEX DNA-Seq Kit.DNA-seq [...]

Can denatured/single-stranded DNA be used with the ThruPLEX DNA-Seq Kit?

No, DNA must be double-stranded for use with the ThruPLEX DNA-Seq Kit.DNA-seq [...]