Does the ThruPLEX DNA-Seq Kit’s coverage span GC-rich regions?

Yes, the ThruPLEX DNA-Seq Kit produces uniform coverage in GC-rich regions.DNA-seq [...]

What is the difference between the ThruPLEX DNA-Seq Kit and other commercially available library preparation kits?

The ThruPLEX DNA-Seq Kit is the fastest and most sensitive library prep kit available. Repair, ligation, and amplification are performed in a single tube in three simple steps. *Input amount: 50 pg to 50 ng *No cleanup steps *Number of steps to library: 3 *Time to library: less than 2 hours *Number of sample transfers: 0DNA-seq [...]

Does Takara Bio have an NGS-ready kit for single-cell applications?

Yes. PicoPLEX Gold DNA-Seq Kits can be used with Illumina NGS platforms.DNA-seq [...]

Can I make the product of the PicoPLEX Single Cell WGA Kit NGS-ready?

Takara Bio does not support this application, but please check the following references for recommended DNA fragmentation sizes: Yang et al. NGS analysis of PicoPLEX WGA-amplified single-copy sorted chromosomes to infer the haplotype of individual human chromosomes. PNAS 108, 12–17 (2011). Leung et al. NGS analysis of single bacteria cells using PicoPLEX WGA amplification on microfluidic [...]

Can the product of the PicoPLEX Single Cell WGA Kit be taken straight to NGS?

No. The PicoPLEX Single Cell WGA Kit does not have adapters nor barcodes. If NGS is the desired application, the WGA material can be used in a library preparation technology (e.g., the ThruPLEX DNA-Seq Kit).DNA-seq [...]