What types of viral vectors can be used with RetroNectin reagent?

RetroNectin reagent can be used to enhance retroviral and lentiviral transduction. It is not known if RetroNectin reagent is useful for introducing nucleic acids.RetroNectin [...]

What cell types can RetroNectin reagent be used with?

RetroNectin reagent is highly effective for cells that express integrin VLA-4 or VLA-5. These integrin molecules can interact with the CS-1 site and the cell-binding domains of Fibronectin. VLA-4-expressing cells include T cells, B cells, monocytes, NK cells, eosinophils, bone marrow monocytic cells, and lymphoid progenitors. Thymocytes, activated T-cells, and mast cells express [...]

What are Very Late Antigen-4 (VLA-4) and Very Late Antigen-5 (VLA-5)?

VLA-4 and VLA-5 belong to the integrin superfamily and are also known as integrin ?4?1 and integrin ?5?1, respectively. Integrins are cell-surface receptors that mediate cell-cell and cell-extracellular matrix interactions.RetroNectin [...]

How does RetroNectin reagent colocalize viral particles and cells? Where do the virus particles bind?

RetroNectin reagent can enhance retroviral-mediated gene transfer into mammalian cells by colocalizing retrovirus particles and target cells. Colocalization is thought to be accomplished by direct binding of viral particles to sequences in the heparin-binding domain, and interaction of cellular integrins VLA-4 and/or VLA-5 with the RetroNectin molecule. RetroNectin [...]

What is Fibronectin?

Fibronectin is a high molecular weight (220–250 kD) component of the extracellular matrix. Fibronectin exists as a dimer and interacts with extracellular matrix components to participate in cell migration, morphology, growth, and adhesion.RetroNectin [...]