What is RetroNectin reagent?

RetroNectin reagent is a recombinant human fibronectin fragment (rFN-CH-296; 574 amino acids; ~63 kD) that contains three functional domains: a cell-binding domain, a heparin-binding domain, and a CS-1 sequence. RetroNectin reagent enhances lentiviral- and retroviral-mediated gene transduction by aiding the colocalization of target cells and viral [...]

What are CFU?

CFU (colony forming units) determine the virus' ability to transduce cells and express protein. Cells harboring integrated proviral DNA and carrying an antibiotic resistance gene survive the presence of the drug and form cell colonies. Lentiviral titer in CFU/ml is determined by counting drug-resistant cell colonies.Retrovirus [...]

What are IFU?

IFU (infectious units) indicate the ability of the virus to infect cells and express protein. You can determine the infectious viral titer (IFU/ml) by measuring the expression of a reporter or a specific protein by flow cytometry, immunostaining, etc. Retrovirus [...]

What is MOI?

MOI (multiplicity of infection) is the number of infectious viral units per cell at the time of infection. Retrovirus [...]

Can I use the Retro-X qRT-PCR Titration Kit to titer MSCV-based retroviruses?

No. The Retro-X qRT-PCR Titration Kit is not compatible with MSCV-based retroviruses. It does, however, work with all MMLV-based retroviral vectors.Retrovirus [...]