SMARTer Ultra low kits: what RNA purification kits are recommended?

Choose the most suitable RNA purification kit for your starting material (e.g., plant, tissue, mammalian cells). The RNA purification kit should be compatible with downstream cDNA synthesis and sequencing, the use of a carrier is not recommended.Note: Some plant species have high levels of polysaccharides, which may be retained in the final RNA prep. Excess polysaccharides may block the primer [...]

What methods can I use to assess RNA quality and quantity?

To determine the RIN and RNA quantity we suggest using the Agilent RNA 6000 Pico Kit (Agilent, Cat. # 5067-1513). If total RNA is not limiting, you may use the Agilent RNA 6000 Nano Kit (Agilent, Cat. # 5067-1511). In our hands, the Agilent RNA 6000 Pico Kit was more accurate for assessing RNA quantity at lower concentrations compared to other assays tested.General [...]

What are the requirements for RNA quality and quantity when using SMARTer Ultra Low kits?

SMARTer Ultra low kits utilize an oligo(dT) primer for first-strand cDNA synthesis and require 10 pg–10 ng input RNA with a RIN >8 to ensure selective and efficient full-length cDNA synthesis from mRNAs.Note: DNase treatment or removal of rRNA from a total RNA prep is not required for SMARTer Ultra low kits.General [...]

What are RNA sample preparation requiremtns for SMARTer Ultra low & SMARTer universal kits

Illumina indexes and adapters are integrated during cDNA amplification with the SMARTer Stranded RNA-Seq Kit (including the HT version) and the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian. No additional library preparation is needed. (see ""Which indexes are included in SMARTer stranded kits?""). Not all indexes can be pooled together, consult the Illumina literature (such as the [...]

What SMARTer cDNA synthesis kits are compatible with Ion Torrent sequencing platforms?

Currently, the SMART-Seq mRNA-seq Kit for Sequencing, the SMARTer Ultra Low Input RNA Kit for Sequencing - v3, and the SMARTer Universal Low Input RNA Kit for Sequencing are compatible with downstream Ion Torrent library preparation and sequencing. General [...]