What are RNA sample preparation requiremtns for SMARTer Ultra low & SMARTer universal kits
What are RNA sample preparation requiremtns for SMARTer Ultra low & SMARTer universal kits
Illumina indexes and adapters are integrated during cDNA amplification with the SMARTer Stranded RNA-Seq Kit (including the HT version) and the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian. No additional library preparation is needed. (see ""Which indexes are included in SMARTer stranded kits?""). Not all indexes can be pooled together, consult the Illumina literature (such as the ""TruSeq® DNA Sample Preparation Guide"") for appropriate pooling guidelines. When in doubt about compatibility, compare the index sequences provided in the user manuals with Illumina adapter sequences.